Journal: BMC Immunology

Article Title: Macrophage-targeted anti-CCL2 immunotherapy enhances tumor sensitivity to 5-fluorouracil in a Balb/c-CT26 murine colon carcinoma model measured using diffuse reflectance spectroscopy

doi: 10.1186/s12865-022-00493-5

Figure Lengend Snippet: Timeline for study. Daily DRS measurements were taken after tumor volumes reached 75 mm 3 up until endpoint analysis (Day 3 or 12). Therapy injections were given on days 0 to 10. 5-FU treated mice were given daily injections while the other treatment groups (saline and isotype controls and anti-CCL2) were given injections every other day. The combination group followed the treatment schedule of the anti-CCL2 and 5-FU groups. Figure was created with BioRender.com

Article Snippet: Anti-CCL2 (Bio X Cell, 2H5, #BE0185) was shipped at 7.4 mg/mL in PBS and stored at 4 °C before injection.

Techniques:

Journal: BMC Immunology

Article Title: Macrophage-targeted anti-CCL2 immunotherapy enhances tumor sensitivity to 5-fluorouracil in a Balb/c-CT26 murine colon carcinoma model measured using diffuse reflectance spectroscopy

doi: 10.1186/s12865-022-00493-5

Figure Lengend Snippet: Longitudinal comparisons in oxygen saturation. Each panel shows the comparison of oxygen saturation in comparison to the saline and isotype control. A Isotype control, B anti-CCL2, C 5-FU and D Combination. A mixed effects model was used to calculate statistical differences (* p ≤ 0.05). Plots created in Prism (GraphPad)

Article Snippet: Anti-CCL2 (Bio X Cell, 2H5, #BE0185) was shipped at 7.4 mg/mL in PBS and stored at 4 °C before injection.

Techniques:

Journal: BMC Immunology

Article Title: Macrophage-targeted anti-CCL2 immunotherapy enhances tumor sensitivity to 5-fluorouracil in a Balb/c-CT26 murine colon carcinoma model measured using diffuse reflectance spectroscopy

doi: 10.1186/s12865-022-00493-5

Figure Lengend Snippet: Longitudinal comparisons in total hemoglobin content. Each panel shows the comparison of oxygen saturation in comparison to the saline and isotype control. A Isotype control, B anti-CCL2, C 5-FU and D Combination. A mixed effects model was used to calculate statistical differences (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001). Plots created in Prism (GraphPad)

Article Snippet: Anti-CCL2 (Bio X Cell, 2H5, #BE0185) was shipped at 7.4 mg/mL in PBS and stored at 4 °C before injection.

Techniques:

Journal: BMC Immunology

Article Title: Macrophage-targeted anti-CCL2 immunotherapy enhances tumor sensitivity to 5-fluorouracil in a Balb/c-CT26 murine colon carcinoma model measured using diffuse reflectance spectroscopy

doi: 10.1186/s12865-022-00493-5

Figure Lengend Snippet: The addition of anti-CCL2 to 5-FU shows a change in hypoxia. A Hypoxia quantification through IHC quantification using CA-IX (10X objective, 0.3NA, scale bars are 50 μm). B The average hypoxic area was calculated per FOV (n = 27 FOVs per group). A mixed effects model was used to calculate statistical differences. Plot created in Prism (GraphPad)

Article Snippet: Anti-CCL2 (Bio X Cell, 2H5, #BE0185) was shipped at 7.4 mg/mL in PBS and stored at 4 °C before injection.

Techniques:

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Efficacy of Candida dubliniensis and Fungal β-Glucans in Inducing Trained Innate Immune Protection Against Inducers of Sepsis

doi: 10.3389/fcimb.2022.898030

Figure Lengend Snippet: Role of macrophages and Gr-1+ leukocytes in C. dubliniensis -induced protection against polymicrobial C. albicans / E. coli IAI. Mice (n = 10/group) were immunized by i.p. injection with 1.75x10 7 CFUs of live C. dubliniensis 14 days prior to lethal challenge. For macrophage depletion, mice were injected i.p. with liposome-encapsulated clodronate or control empty liposomes 1 day prior to lethal challenge. For Gr-1+ cell depletion, mice were injected i.p. with 200 μg anti-Gr-1 or isotype control antibody 48 h prior to and 2 h after lethal challenge. Mice were challenged with C. albicans (1.75x10 7 CFUs) and E. coli (4.5x10 6 CFUs) i.p. and monitored for (A) survival and (B) morbidity/sepsis scoring for 10 days post-lethal challenge. Naïve (unvaccinated) mice served as the negative control. Graphs are representative of 2 separate experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 [for values significantly different from those of the control, by log rank Mantel-Cox test (survival) and ANOVA followed by post hoc Student’s t-test (sepsis scoring)].

Article Snippet: For granulocyte depletion, mice were injected i.p. with either 200 μg rat anti-mouse Gr-1 + (Ly6G/Ly6C) or rat IgG2A isotype control antibodies (Bio-X-Cell) in 200 µl sterile non-pyrogenic PBS to systemically deplete PMNLs 48 h prior to and 2 h after challenge.

Techniques: Injection, Control, Liposomes, Negative Control

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Efficacy of Candida dubliniensis and Fungal β-Glucans in Inducing Trained Innate Immune Protection Against Inducers of Sepsis

doi: 10.3389/fcimb.2022.898030

Figure Lengend Snippet: Role of macrophages and Gr-1+ leukocytes in C. dubliniensis -induced protection in zymosan-induced sepsis. Mice (n = 10/group) were immunized by i.p. injection with 1.75x10 7 CFUs of live C. dubliniensis 14 days prior to lethal challenge. For macrophage depletion, mice were injected i.p. with liposome-encapsulated clodronate or control empty liposomes 1 day prior to lethal challenge. For Gr-1+ cell depletion, mice were injected i.p. with 200 μg anti-Gr-1 or isotype control antibody 48 h prior to and 2 h after lethal challenge. Mice were challenged with 700-1000 mg/kg zymosan and monitored for (A) survival and (B) morbidity/sepsis scoring following lethal challenge for 10 days. Naïve (unvaccinated) mice served as the negative control. Graphs are cumulative data from 3 separate experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 [for values significantly different from those of the control, by log rank Mantel-Cox test (survival) and ANOVA followed by post hoc Student’s t-test (sepsis scoring)].

Article Snippet: For granulocyte depletion, mice were injected i.p. with either 200 μg rat anti-mouse Gr-1 + (Ly6G/Ly6C) or rat IgG2A isotype control antibodies (Bio-X-Cell) in 200 µl sterile non-pyrogenic PBS to systemically deplete PMNLs 48 h prior to and 2 h after challenge.

Techniques: Injection, Control, Liposomes, Negative Control

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Efficacy of Candida dubliniensis and Fungal β-Glucans in Inducing Trained Innate Immune Protection Against Inducers of Sepsis

doi: 10.3389/fcimb.2022.898030

Figure Lengend Snippet: Role of macrophages and Gr-1+ leukocytes in β-glucan-induced protection against lethal polymicrobial C. albicans/S. aureus IAI. Mice (n= 10/group) were immunized with either modified β-glucan (A, B) or d-zymosan (C, D) prior to lethal challenge as described in Figure 5 . For macrophage depletion, mice were injected i.p. with liposome-encapsulated clodronate or control empty liposomes 1 day prior to lethal challenge. For Gr-1+ cell depletion, mice were injected i.p. with 200 μg anti-Gr-1 or isotype control antibody 48 h prior to and 2 h after lethal challenge. Mice were challenged with 700-1000 mg/kg zymosan and monitored for (A, C) survival and (B, D) morbidity/sepsis scoring following lethal challenge for 10 days. Naïve (unvaccinated) mice served as the negative control. Graphs are representative of at least 3 separate experiments. The exception is (A) that shows cumulative data of 3 separate experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 [for values significantly different from those of the control, by log rank Mantel-Cox test (survival) and ANOVA followed by post hoc Student’s t-test (sepsis scoring)].

Article Snippet: For granulocyte depletion, mice were injected i.p. with either 200 μg rat anti-mouse Gr-1 + (Ly6G/Ly6C) or rat IgG2A isotype control antibodies (Bio-X-Cell) in 200 µl sterile non-pyrogenic PBS to systemically deplete PMNLs 48 h prior to and 2 h after challenge.

Techniques: Modification, Injection, Control, Liposomes, Negative Control